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刊物期号: 201809

高效液相色谱-串联质谱法同时测定酿酒原料中10种真菌毒素(86)

作者: 周韩玲,廖勤俭,宋廷富,安明哲,李杨华,王 芳,郭 艳,王小琴,罗 珠

地址:宜宾五粮液集团有限公司技术中心,四川 宜宾 邮编:644007
摘要 建立酿酒原料中10种真菌毒素的高效液相色谱-串联质谱(LC-MS/MS)检测方法。样品经84 %乙腈提取后,采用黄曲霉毒素免疫亲和柱净化、外标法定量4种黄曲霉毒素;使用多功能柱净化、同位素内标法定量样品中另外6种真菌毒素。结果表明,10种真菌毒素在各自的线性范围内线性关系良好,相关系数不低于0.998,10种化合物检出限为0.0022~1.79 μg/L,定量限为0.075~5.95 μg/L;加标回收率为61 %~116.2 %,相对标准偏差为0.88 %~8.61 %。该方法具有灵敏度高、稳定性好、定量准确的特点,适用于不同酿酒原料中多种真菌毒素的定量检测。
关键词 高效液相色谱-串联质谱; 酿酒原料; 真菌毒素; 多功能柱; 免疫亲和柱
中图分类号: TS262.3;TS261.2;TS261.7 文献标识码: A 文章编号: 1001-9286(2018)09-0086-06

英文标题: Simultaneous Determination of 10 Kinds of Mycotoxins in Liquor-Making Raw Materials by HPLC-MS/MS

英文作者: ZHOU Hanling,LIAO Qinjian,SONG Tingfu,AN Mingzhe,LI Yanghua,WANG Fang,GUO Yan, WANG Xiaoqin and LUO

英文地址: Technology Center of Wuliangye Group Co. Ltd., Yibin, Sichuan 644007, China

英文摘要 A method for simultaneous determination of 10 kinds of mycotoxins in liquor-making raw materials by HPLC-MS/MS had been developed. The samples were extracted with 84 % acetonitrile. Four kinds of aflatoxins were quantified by external standard method with aflatoxin immunoaffinity column purification, and the other six mycotoxins were quantified by internal standard method with multi-functional column purification. The results showed that the 10 mycotoxins had a good linear relationship in their respective linear range with a correlation coefficient no less than 0.998. The detection limits of the 10 compounds were 0.0022 μg/L to 1.79 μg/L with the limits of quantitation as 0.075 μg/L to 5.95 μg/L. The recoveries were 61 % to 116.2 % with the relative standard deviations as 0.88 % to 8.61 %. This method had the advantages including high sensitivity, good stability and quantitative accuracy, and it was suitable for quantitative detection of various mycotoxins in different liquor-making raw materials.
英文关键词 high performance liquid chromatography-tandem mass spectrometry; liquor-making raw materials; mycotoxins; multifunctional column; immunoaffinity column
      

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