|地址：1. 苏州出入境检验检疫综合技术中心，江苏 苏州 215100； 2. 苏州世标检测技术有限公司，江苏 苏州 215100
建立了高效液相色谱串联质谱仪对酒类产品中11 种PDE-5抑制剂及其衍生剂的快速分析方法。样品经乙腈∶水（1∶1，v/v）超声提取，Atlantis d C18色谱柱(2.1×150 mm，3 μm)为分析柱，0.1 %甲酸溶液和0.1 %甲酸乙腈为流动相，电喷雾正离子多反应监测模式进行检测，外标法定量。试验结果表明，该方法的RSD 值范围为0.57 %～11.69 %，回收率范围在60.0 %～118.6 %，该方法的线性相关性良好，检测低限、精密度和准确度均符合要求，能够满足酒类产品中11种PDE-5抑制剂及其衍生剂的检测要求。 |
液质联用仪； 酒类； PDE-5抑制剂 |
Determination of 11 Kinds of PDE-5 Inhibitors and their Derivatives in Alcoholic Drinks by LC-MS/MS
1. Suzhou Entry-Exit Inspection and Quarantine Bureau, Suzhou, Jiangsu 215100; 2.Suzhou World Standard Testing Technology Co. Ltd., Suzhou, Jiangsu 215100, China
A method of high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) had been developed for the determination of 11 kinds of PDE-5 inhibitors and their derivatives in alcoholic drinks. Samples were diluted with acetonitrile∶water (1∶1, v:v) and the analyte was separated by an Atlantis dC18 column （2.1×150 mm, 3 μm）using a mobile phase consisting of 0.1 % formic acid and acetonitrile with 0.1 % formic acid. The analyte was detected in multiple-reaction monitoring (MRM) mode via positive electrospray ionization (ESI+). The determination results showed that the RSD was between 0.57 % to 11.69 % and the recovery was between 60.0 % to 118.6 % at three spiked levels. The method was characterized by its excellent purification effect, high sensitivity and good accuracy, and it could be used for the determination of 11 kinds of PDE-5 inhibitors and their derivatives in alcoholic drinks.
high performance liquid chromatography tandem mass spectrometry (LC-MS/MS); alcoholic drinks; PDE-5 inhibitor