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刊物期号: 201705

窖泥中产丁酸菌的筛选、鉴定及生长性能研究(35)

作者: 袁华伟1,赵 猛1,蒋 君1,刘光钱2,胡永奇2,3,沈才洪2,3,吴重德4,周荣清3,4,宋 川2,3

地址:1.宜宾学院生命科学与食品工程学院,四川 宜宾 644000; 2.泸州老窖股份有限公司,四川 泸州 646000; 3.国家固态酿造工程技术研究中心,四川 泸州 646000; 4.四川大学轻纺与食品学院,四川 成都 610065
摘要 通过对川南某酒厂的老窖泥富集培养、分离纯化,经丁酸发酵实验,筛选出1株高产丁酸的梭菌。经16Sr DNA鉴定,该菌为丁酸梭状芽孢杆菌(Clostridium butyricum),命名为丁酸菌YD-4。经对该菌株生长性能条件研究,其最适生长温度为37 ℃,最适pH值为7.0,最适接种量为2 %,菌体浓度达1.64×108个/mL,产丁酸量可达到3.44 g/L。
关键词 窖泥; 丁酸菌; 筛选; 鉴定; 生长性能
中图分类号: TS262.3;TS261.4;TS261.1 文献标识码: A 文章编号: 1001-9286(2017)05-0035-06

英文标题: Isolation and Identification of a Butyric Acid-Producing Strain from Pit Mud and Study on Its Growth Performance

英文作者: YUAN Huawei1,ZHAO Meng1,JIANG Jun1,LIU Guangqian2,HU Yongqi2,3,SHEN Caihong2,3, WU Chongde4,ZHOU Ron

英文地址: 1.College of Life Science and Food Engineering,Yibin University,Yibin,Sichuan 644000; 2.Luzhou Laojiao Co. Ltd.,Luzhou,Sichuan 646000;3.National Engineering Technology Research Center of Solid-state Brewing,Luzhou,Sichuan 646000;4.College of Light In

英文摘要 A Clostridium strain with high yield of butyric acid was isolated through cumulation cultivation, isolation, purification, and butyric acid fermentation from aged pit mud in a distillery in south Sichuan. 16Sr DNA identification suggested that such strain is Clostridium butyricum and it was named YD-4. The research on its growth performance indicated that its optimum growth temperature was at 37 ℃, its optimum pH value was about 7.0, its best inoculating quantity was 2 %. As the total number of bacteria reached up to 1.64×108个/mL, the yield of butyric acid was 3.44 g/L.
英文关键词 pit mud; butyric acid-producing bacteria; isolation; identification; growth performance
      

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