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刊物期号: 200403

红葡萄酒中酚类化合物的快速高效液相色谱分析

作者: M.Ibern-Gomezet al著朱立红1,赵荣华2,周洪福1

地址:1.张裕葡萄酿酒股份有限公司葡萄酒分公司;2.张裕集团技术中心,山东 烟台 264000
摘要 阐述了一种新型的可直接而快速分离红葡萄酒中酚类化合物(苯甲酸、黄烷-3-醇、肉桂酸、黄酮醇、花色素)的反相高效液相色谱法。采用的色谱柱可用于低pH环境且能高流速运行,在均含有0.2 %三氟乙酸的水和乙腈两相中进行梯度洗脱。为了提高选择性,每种化合物都在其最大吸光度处被检测,从而建立其精密度、线性和灵敏度。尽管这种方法不能检测酒中所有组分,但适用于主要成分的测定和对特殊类别酚类化合物总量的定量分析。
关键词 分析; 花色素; 儿茶酸; 直接进样; 类黄酮; 羟基肉桂酸; 液相色谱
中图分类号: O657.72;TS261.7;TS262.6 文献标识码: B 文章编号: 1001-9286(2004)03-0089-02

英文标题: Rapid HPLC Analysis of Phenolic Compounds in Red Wines

英文作者: By M. Ibern-Gomez et al Tran. by ZHU Li-hong et al.

英文地址: Zhangyu Wine Co.Ltd.,Yantai, Shandong 264000, China

英文摘要 A new direct and rapid (18 min) reversed-phase high-performance liquid chromatography method for the separation of phenolic compounds (benzoic acids,flavan-3-ols,cinnamic acids,flavonols,and anthocyanins) in red wines is described. A column that allows for low pH conditions and high flow was used,with a gradient of two solvents:water and acetonitrile,both with 0.2 % trifluoroacetic acid. To improve selectivity, each compound was monitored at its absorbance maximum. Precision, linearity, and sensitivity (limit of detection and limit of quantitation) were established. While this rapid method cannot resolve all wine constituents, it is appropriate for measuring major components and quantifying total amounts of particular classes of phenolic compounds. The method was applied to a set of new and aged red wines.
英文关键词 Analysis; anthocyanins; catechins; direct injection; flavonoids; hydroxycinnamates; liquid chromatography
      

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